In ovo Vaccine Against Infectious Bursal Disease
نویسندگان
چکیده
A recombinant attenuated vaccine against infectious bursal disease virus (IBDV) was administered in ovo to 18-day-old embryos. The vaccine was genetically tailored to protect from challenges in the field against classic and variant strains of IBDV. The vaccine virus contains neutralizing epitopes from both classic (D78) and variant strain (GLS), and abrogates expression of the nonstructural protein, VP5 of IBDV. Specificpathogen-free (SPF) and fertile broiler eggs obtained from a local poultry farm were vaccinated and used to evaluate protection against IBDV-STC challenge. A full dose of the vaccine consisting of 5.6x10 plaque 3 forming units (pfu) was administered to SPF and broiler embryos. In addition, a half dose of the vaccine containing 2.3x10 pfu 21 was injected into SPF embryos. The vaccine had no effect on hatchability or first 3 week survival in either broilers or SPF birds, even when high doses were administered. The vaccine generated high antibody titers in chickens with either dosage. All vaccinated groups were protected against mortality. The vaccine did not cause bursal damage and fully protected SPF chickens vaccinated in ovo with half dose of the vaccine and broiler chicks that received a full dose of the recombinant vaccine in ovo.
منابع مشابه
Pathological and immunological study of an in ovo complex vaccine against infectious bursal disease.
The appearance of very virulent strains of infectious bursal disease (IBD) virus at the end of the 1980s made it necessary to develop more effective immunization procedures. To facilitate this, the immunogenicity and the immunosuppressive effect of a mild (G-87), an intermediate (LIBD) and an intermediate-plus (IBDV 2512) IBDV strain were tested after the in ovo inoculation of 18-day-old SPF an...
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Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-γ
The aim of this study was to examine the efficacy of in ovo prime-boost vaccination against infectious bursal disease virus (IBDV) using a DNA vaccine to prime in ovo followed by a killed-vaccine boost post hatching. In addition, the adjuvant effects of plasmid-encoded chicken interleukin-2 and chicken interferon-gamma were tested in conjunction with the vaccine. A plasmid DNA vaccine (pcDNA-VP...
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By mid-August 1995, 55% of broiler embryos in North America were vaccinated for Marek’s disease using the INOVOJECT system, with 201 INOVOJECT machines placed with 16 of the top 25 poultry producers, providing the industry with the capacity to inject in excess of 400 million eggs per month or about 5 billion eggs per annum. In ovo administration of a bursal disease antibody-infectious bursal ...
متن کاملEvaluation of the Protection against Infectious Bursal Disease (IBD) Challenge in Progeny Born to Parents Having Received a Vaccination Program Using a Herpesvirus of Turkey-Infectious Bursal Disease (HVT-IBD) Vector Vaccine
Broiler breeder vaccination against IBD is usually based on the injection of at least one inactivated vaccine in oil adjuvant, typically included in a combined vaccine. Priming using one or several IBD vaccine (s) has been the most common way to immunize the breeders so far. In summary, protection against vvIBD challenge in chicks of one commercial genetic line vaccinated in ovo with the HVT-IB...
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